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Solute Experiment
Testable Question What solvent or solvent combination will work the best to separate the colors in black using chromatography paper?
Hypothesis If the solvent is alcohol then the colors in black ink will separate more then if they were water.
Materials Chromatography papers (4), Metric ruler, Para film, 50 mL Erlenmeyer flask (), Black felt tip pen, Black Overhead marker, Isopropyl Alcohol, Distilled water, Hydrogen peroxide, pipettes.
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Procedure Precautions Make sure that Ink dots are above the solvent level, Crease the chromatography paper to make sure that is doesn't fall into the solvent.
Chemical Precautions Isopropyl Alcohol is a flammable chemical, make sure there are no open flames and that the area in which you are conducting your experiment is well ventilated, to ensure that you don't inhale the fumes cover the Flasks as soon as you pour the solvent in the flask.
Procedure
1. Turn on the hot tap water and let it run until it is very warm.
. While the water is warming up, take the pice of chromatography paper and draw a pencil line about 1 cm from the bottom edge.
. Take the pen you are testing and make a small but significant dot of ink on the pencil line.
4. Put about ½ cm of warm tap water in the bottom of each flask.
5. Place the chromatography paper in the flask, with the pencil edge at the bottom.
6. Cover the flask with a pice of Para film , being careful not to disturb the liquid and paper in the flask.
7. Wait about 0 minutes while the solvent is drawn up to the top of the paper.
8. When the front edge of the solvent is almost at the top of the chromatography paper, remove the paper, lay it on a paper towel, and with the pencil mark the solvent front.
. Allow the paper to dry. Measure the distance each color moves away from the original pencil line.
10. For the second Experiment for step 1, prepare the Isopropyl Alcohol.
11. Instead of using water for step 4 use Alcohol.
1. Then follow the rest of the steps in this experiment.
chem lab.
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